Heme Polymerization Inhibion Assay (HPIA) of Ethyl acetate fraction of Faloak Bark (Sterculia Quadrifida, R.Br) as antiplasmodial

Taku Bessi, Marce I. and Fernandez, Stefany S.A. (2021) Heme Polymerization Inhibion Assay (HPIA) of Ethyl acetate fraction of Faloak Bark (Sterculia Quadrifida, R.Br) as antiplasmodial. In: Abstract Book ICoCLEH 2020, 8-9 October 2021, Surabaya.

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Abstract

Inhibition of hemoglobin polymerization is one of the main targets of antiplasmodial activity. Previous research revealed that flavonoids had antimalarial activity in vitro and one of the plants that have high flavonoids is Faloak (Sterculia quadrifida R.Br). Flavonoid content in Faloak found highest in ethyl acetate fraction of bark extract. The study aimed to investigate the activity of Ethyl acetate fraction of Faloak Bark (EAFB) in inhibiting heme polymerization. Heme polymerization inhibition assay from Huyen and based on reduction of heme absorption in a β-hematin formation using Tween 20 as an initiator. The inhibitory activity of heme polymerization expressed as IC50 and was determined by probit analysis. Heme polymerization inhibition activity of EAFB and Cloroquin as control were 49,70±15,2 mg/mL and 45,40±4,47 mg/mL respectively. A T-test on IC50 showed no significant difference between EAFB and chloroquine. Conclusion: Faloak bark of ethyl acetate fraction has heme polymerization inhibitory activity.

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